ACE analysis of binding of peptides to low-molecularweight heparin. ACE⁶  was used to study interactions between peptides and low-Mr heparin. Trace concentrations of radiolabeled heparin are electrophoresed through agarose lanes containing proteins at various concentrations. The electrophoretic patterns of radiolabeled heparin are then visualized using a Phosphorimager, and K_ds of peptide-heparin interactions were calculated from binding plots. Per peptide, 4 assays were performed. Results of all the experiments are shown in Table 1. The panels show the Phosphorimager tracings of the ACE gels, and the concentration of peptide used in each lane is shown.