Migration of PDCs to adenosine or chemokines. (A) Dose-response curve for the migration of freshly isolated PDCs toward adenosine. Mean ± SEM of 7 experiments, each performed in duplicate, are shown. (B) Dose-response curve for the migration of PDCs cultured overnight in the presence of IL-3 toward adenosine. Mean ± SEM of 3 experiments, each performed in duplicate, are shown. (C) Migratory response of PDCs toward adenosine, CXCL12 (SDF-1α), or CCL21 (6Ckine) after isolation (ex vivo) and after 1 day of culture with IL-3. Mean ± SEM of 4 to 6 independent experiments, each performed in duplicate, are shown. (D) PDCs were cultured overnight in the presence of IL-3 or for 48 hours in the presence of IL-3 and CD40L trimer before the assessment of their migratory response to adenosine. Mean ± SEM of duplicates are shown.