Mixed lymphocyte reaction (MLR) and proliferation and IFN-γ production by autologous CML T lymphocytes after coculture with DCs generated in the presence of IFN-α: effect of LPS. Monocytes from CML patients were treated with either IFN-α/GM-CSF or IL-4/GM-CSF for 3 days. Cell cultures were further incubated for 20 hours in the presence or absence of LPS. (A) The proliferation of allogeneic T cells cocultured with the different DC populations at the indicated ratios. After 5 days of coculture, [³H]thymidine was added for 18 hours, and the cells were analyzed for thymidine uptake (^*P < .01, ^**P < .05). (B) The proliferation of autologous T cells stimulated by the different DC populations as determined by a [³H]thymidine assay (^*P < .01). The results in panels A and B represent the mean ± SE of data obtained from 3 single cultures. (C) The IFN-γ production by DC-stimulated autologous T cells. Supernatants from cocultures of DCs, generated as previously indicated, and autologous T cells were harvested after 6 days and tested for IFN-γ production by ELISA. (B-C). The single values are subtracted from the relative controls represented by basal [³H]thymidine incorporation and spontaneous IFN-γ production of DCs cultured alone. The values of spontaneous proliferation of T cells were under 300 cpm, and no IFN-γ production was observed in control cultures. (D) CD83 expression of the DC populations used in the coculture experiments illustrated above. Representative data obtained with cells from 1 patient out of 4 are shown.