RT-PCR confirmation of specific microarray results. RT-PCR was performed on 0.5-μg samples of total bone marrow RNA. (A-B) The serpin-protease inhibitor PAI-2, at 25 and 30 cycles, respectively. (B-G) Receptors IL-5Rα, CCR1, CCR2, CCR3, and CCR5. (H) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control. All RT-minus control reactions were negative. Far right lane, positive controls as indicated.