Monocyte-derived DCs from chronic HCV patients undergo normal TNF-α-dependent maturation. (A-B) Representative examples of iDCs (A) and mDCs (B) from chronic HCV patient no. 3. Geometric mean fluorescence intensity (MFI) is indicated. See Supplementary Table S1 for numeric values and additional data on all patients. (C) Representative examples of mDCs from a noninfected (NI) donor and a patient with chronic HCV. Expression of the indicated surface marker (dark lines) is overlaid with the isotype control (dotted lines). All antibodies were obtained from BD Biosciences. (D) The mDCs from chronic HCV patient no. 936 were adhered to Alcian blue-treated coverslips, fixed with 3.4% paraformaldehyde (PFA), and stained with anti-MHC II (clone 93C9), followed by secondary antimouse immunoglobulin G (IgG) Alexa 488. Cells were visualized with a Zeiss Axioplan 200. Original magnification, × 630.