DALD patients display increased serum levels of OPN. (A) Autoradiographs of macroarray membranes hybridized with cDNA from a DALD patient (i) and from her healthy brother (ii). Bottom spots show OPN and top spots show oncostatin M as a control. (B) Serum concentrations of OPN (ng/mL) in 25 DALD patients and 50 healthy unrelated controls. The bold horizontal bars indicate the median values for each group; the dotted line, the 95th percentile of the controls. The P value of differences between patients and controls was calculated using the Mann-Whitney U test. (C) Correlation between OPN and IgG serum levels in DALD patients. The line is the best fitting regression line. The r and P values were evaluated using the Pearson correlation analysis. OPN levels were evaluated by ELISA.