In vitro reactivation of Ad-specific CTL lines. The cell lines, from donors 1 (A) and 12 (B), were generated by PBMC stimulation with Ad5f35GFP-transduced DCs. In panels Ai and Bi, ELISPOT assays show the relative specificities of the CD4 and CD8 T cells. T cells were incubated with autologous LCL targets (auto) either alone or transduced with Ad5f35GFP (auto + Ad); negative controls were class I-mismatched LCLs alone (mis) or Ad5f35GFP transduced (mis + Ad). Results are expressed as SFCs per 5 × 10³ cells. Panels Aii, Aiii, Bii, and Biii display the ability of these expanded T cells to kill autologous Ad-transduced targets in a standard chromium release assay using the same targets as described in the ELISPOT assay. Results are expressed as percent specific lysis of autologous LCLs alone (♦), allogeneic LCLs alone (▪), Ad5f35GFP-transduced autologous LCLs (▴), or Ad5f35GFP-transduced allogeneic LCLs (x). Panels Aii and Bii show isolated CD4⁺ T-cell cytotoxicity assay results, and panels Aiii and Biii show CD8-isolated T cells.