Effect of HO-1 induction on inflammatory angiogenesis in vivo. Matrigel containing LPS (10 ng/mL) plus CoPP (25 μM) was injected subcutaneously into C57BL/6 mice. After 6 days plugs were explanted and fixed in formalin and paraffin embedded. Quantification of neovascularization was performed on hematoxylin and eosin–stained sections as described in “Materials and methods.” The results are expressed as percentage ± SE of the vessel area with respect to the total Matrigel area. Nine mice were used per condition in each experiment. ANOVA with Dunnett multicomparison test was performed between LPS and LPS plus CoPP (*P < .05).