Figure 4.
Effect of IFN-γ on granzyme B and perforin mRNA. (A) Highly purified PMNs (106) were incubated with IFN-γ (100 U/mL) for the times indicated; then, mRNA was extracted and RT-PCR was performed with primers specific for either perforin or granzyme B. β-actin was used as a housekeeping gene. (B) Semiquantitative analysis using the data derived from scanning corrected for the β-actin data (nonstimulated PMNs, □; IFN-stimulated PMN, ▪).
