American Society of Hematology

Figure 1.

$Figure 1. Sequence and GeneScan analysis of DNA from purified lymphocyte fractions. (Ai) WASP exon 10 genomic sequence surrounding the 1305insG mutation. DNA was isolated from sorted, WASP-negative T cells3 of patient II-1. The original 1305insG mutation is highlighted by a red box, and the DraII recognition sequence is underlined. (Aii) Sequence analysis of the same genomic region as in (i) performed on DNA isolated from sorted, WASP-positive T cells and showing the presence of Δ19bp. (Aiii) Sequence of the gel-purified Δ19bp fragment amplified from DNA obtained from sorted B cells after DraII digestion. (B) GeneScan analysis of specific 1305insG (insG) and Δ19bp (Δ19) fragments obtained from DNA isolated from purified CD3+ T cells and CD20+ B cells before and after digestion with DraII.$

Sequence and GeneScan analysis of DNA from purified lymphocyte fractions. (Ai) WASP exon 10 genomic sequence surrounding the 1305insG mutation. DNA was isolated from sorted, WASP-negative T cells³ of patient II-1. The original 1305insG mutation is highlighted by a red box, and the DraII recognition sequence is underlined. (Aii) Sequence analysis of the same genomic region as in (i) performed on DNA isolated from sorted, WASP-positive T cells and showing the presence of Δ19bp. (Aiii) Sequence of the gel-purified Δ19bp fragment amplified from DNA obtained from sorted B cells after DraII digestion. (B) GeneScan analysis of specific 1305insG (insG) and Δ19bp (Δ19) fragments obtained from DNA isolated from purified CD3⁺ T cells and CD20⁺ B cells before and after digestion with DraII.

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