Cytokine profiles reflect both CD4⁺ and CD8⁺ T-cell responses. Cytokine profiles obtained from CD4⁺ and CD8⁺ responder T cells during SCT donor-antipatient MLRs. MLRs were performed using highly purified (> 95%) CD4⁺ or CD8⁺ donor T-cell responders against whole PBMC patient stimulator cells. Supernatants were harvested on day 7 of the MLR and assessed for IFN-γ, IL-5, and IL-13 levels using ELISA. Data are expressed as mean of duplicate cultures with standard error of the mean less than 10% for each point. Three typical responses are shown from a total of 12 experiments performed.