Effect of potential inhibitors on adhesion of murine HS/HE RBCs to human TSP. (A) Washed RBCs from mice and humans with HS or HE (as described in Figures 1-2) were preincubated with control buffer (▪), 500 μg/mL high–molecular weight dextran sulfate (□), 500 μg/mL chondroitin sulfate A (▤), or 10 μg/mL phosphatidylserine-binding annexin V (▧) at 37 °C for 30 minutes prior to perfusion through flow chambers coated with human TSP. (B) Washed RBCs from mice and humans with HS or HE (as described in Figures 1-2) were preincubated with control buffer (▪), antimouse CD47 clone miap301 (□, 2 μg/mL), antihuman CD47 clone B6H12 (▤, 2 μg/mL), antihuman CD47 clone 1F7 (▦,2 μg/mL), or 200 μM TSP peptide 4N1K (▧) at 37°C for 30 minutes prior to perfusion through flow chambers coated with human TSP. Adherent RBCs/mm² in the presence of potential inhibitors are normalized to adherent RBCs/mm² in control buffer. Results are mean ± SEM for n = 3 samples; *P < .001 versus control buffer for panel A and P < .05 versus control buffer for panel B.