Figure 3.
Figure 3. CIITA inhibits the Tax-2-mediated HTLV-2 LTR transactivation. HeLa cells were cotransfected with a fixed amount of pLTR II-Luc1.1 plasmid (LTR luc, 20 ng), a fixed amount of pcDNA3.1-tax2b (tax, 100 ng DNA), and increasing amounts of pcfCIITA1-1130, or pcfCIITA1-321, or pcfCIITA322-1130 plasmid DNA. Viral promoter transactivation was measured as reported in “Materials and methods” and expressed as relative LTR transactivation in the ordinate. Bars represent standard error of triplicate values. The expression of FLAG-tagged CIITA proteins was assessed by Western blotting with an anti-FLAG antibody (WB αFLAG).

CIITA inhibits the Tax-2-mediated HTLV-2 LTR transactivation. HeLa cells were cotransfected with a fixed amount of pLTR II-Luc1.1 plasmid (LTR luc, 20 ng), a fixed amount of pcDNA3.1-tax2b (tax, 100 ng DNA), and increasing amounts of pcfCIITA1-1130, or pcfCIITA1-321, or pcfCIITA322-1130 plasmid DNA. Viral promoter transactivation was measured as reported in “Materials and methods” and expressed as relative LTR transactivation in the ordinate. Bars represent standard error of triplicate values. The expression of FLAG-tagged CIITA proteins was assessed by Western blotting with an anti-FLAG antibody (WB αFLAG).

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