American Society of Hematology

Figure 3.

$Figure 3. Effect of fractalkine on platelet adhesion to collagen. Firm adhesion of washed human platelets to collagen was assessed in a flow chamber model under high (A) and low (B) shear following stimulation with rhCX3C (1 μg/mL) or ADP (5 μM) in the presence or absence of apyrase (7.5 U/mL). The proadhesive effect was blocked by preincubation with an antagonizing antibody against fractalkine (AF537; 100 μg/mL; 30 min) as well as by disruption of CX3CR1-mediated signaling with PTX (0.4 nM; 45 min). Data are expressed as the ratio of adhering platelets to the control group (□) ± SEM from at least 5 separate experiments. **P < .01 versus control; ##P < .01 versus stimulation.$

Effect of fractalkine on platelet adhesion to collagen. Firm adhesion of washed human platelets to collagen was assessed in a flow chamber model under high (A) and low (B) shear following stimulation with rhCX₃C (1 μg/mL) or ADP (5 μM) in the presence or absence of apyrase (7.5 U/mL). The proadhesive effect was blocked by preincubation with an antagonizing antibody against fractalkine (AF537; 100 μg/mL; 30 min) as well as by disruption of CX₃CR1-mediated signaling with PTX (0.4 nM; 45 min). Data are expressed as the ratio of adhering platelets to the control group (□) ± SEM from at least 5 separate experiments. **P < .01 versus control; ##P < .01 versus stimulation.

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