Pathologic study of GvHD in the digestive tract. (A) The superficial epithelium of a villous is split from the lamina propria, with a large area of subepithelial edema. Hematoxylin-eosin, original magnification × 400. (B) Immunostaining with an antibody directed against CD95. Cells located in the glandular epithelium (arrows) as cells located in the lamina propria are stained. Indirect immunoperoxydase, original magnification × 800. (C-D) Immunostaining with an antibody directed against TNF. (C) Numerous stained cells are found in the upper part of the lamina propria (arrows). Indirect immunoperoxidase, original magnification × 250. (D) Full split of the superficial epithelial layer; the cells expressing TNF are mainly located in the split epithelium (arrowheads) or just beneath (arrow). Indirect immunoperoxidase, original magnification × 40. (E-F) TUNEL reactions. (E) Apoptotic bodies (arrows) are mainly located in the upper part of the lamina propria, in close vicinity to the edematous area; original magnification × 250. (F) Apoptotic bodies are mainly found in the lamina propria (arrows), but few apoptotic bodies are also found in the glandular epithelium (arrowheads); original magnification × 600.