In vivo CD4 depletion of BMCs and DLIs administered on day 0 facilitate full donor myeloid and lymphoid engraftment. (A-F) Time course of individual MAC-1⁺ donor myeloid cell (left panels) and CD3⁺ donor T cell (right panels) chimerism in WBCs of B6 (H2^b) mice treated with CTX 200 mg/kg on day –1 and 7 Gy thymic irradiation on day 0 followed by 15 × 10⁶ BMCs and 10 × 10⁶ SPCs on day 0 from B10.A (H2^a) donor mice. Five days before BMT, recipients were given anti-CD4 mAb alone (n=6; A-B), anti-CD8 mAb alone (n=6; C-D), or anti-CD4 and anti-CD8 mAbs (n=5; E-F). Each line represents an individual animal in each panel. One of 2 experiments is presented.