Angiogenesis in CAMs induced by CCL16-Cos. CAMs were recorded at day 12 of incubation, 96 hours after the implant of a gelatin sponge soaked with Vec-Cos (A), CCL16-Cos at 100 ng (B) or 50 ng (C), or with CCL16-Cos (100 ng) neutralized by an Ab anti-CCL16 (D). Panels E and F show the CAM vascularization induced by BX471 (1 μmol/sponge) alone (E) or combined with CCL16-Cos (100 ng/sponge). Arrows in B and in C indicate the new capillaries attracted by the sponge containing CCL16. Original magnifications × 250. The picture is representative of one experiment of at least 4 performed.