HUVEC morphogenesis induced by CCL16. Human HUVECs (2 × 10⁴) were plated onto matrigel deprived of growth factors and stimulated as indicated. After 14 hours of incubation the cell's 3-dimensional organization was recorded (original magnification × 10). (Ai) HUVECs stimulated with Vec-Cos; (ii) ECs stimulated with CCL16-Cos (50 ng/mL); (iii) HUVECs stimulated with CCL16-Cos (50 ng/mL) neutralized by Ab anti-CCL16; (iv) HUVECs stimulated with CCL16-Cos (50 ng/mL) treated with goat IgG; (v) HUVECs preincubated with BX417 (10^–7 M) and then stimulated with CCL16-Cos; (vi) HUVECs preincubated with BX417 (10^–8 M) for 15 minutes and then stimulated with CCL16-Cos (50 ng/mL); (vii) HUVECs preincubated with Ab anti-CCR8 then stimulated with CCL16-Cos (50 ng/mL); (viii) HUVECs stimulated with VEGF-A₁₆₅ (20 ng/mL). Results representative of 3 independent experiments. (B) Morphometric analysis of the above-indicated conditions expressed as % of variation assuming VEGF-A₁₆₅ treated HUVECs as 100% (Aviii). Mean ± SD of 4 independent experiments.