Figure 9.
![Figure 9. Cross-linking of [125I]CCL16 to HUVECs. HUVECs (2 × 107) were cross-linked with 0.5 nM [125I]CCL16 in presence or absence of 100-fold unlabeled ligand as detailed in “Materials and methods.” At the end of incubation, cell lysate was immunoprecipitated by a polyclonal Ab anti-CCR1 and protein separated by SDS-PAGE (10%). The figure is representative of 3 experiments performed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/1/10.1182_blood-2003-05-1387/6/h80145410009.jpeg?Expires=1724139526&Signature=0-Bmaw5Q8yyhbl~7tNzhHQfVyPm4Ae3EIukyjkzi6CMygWUIv5QuiFHyB2VM9x5XZLi~BooVFnbeTSXOXEYzxsx5oNxKsSBSstoE32BAzYUZ~X5-0kiixPssXe3tPgd~8uG6cYGU-y5cSxBm9b9XbFwmvwOYCOgyEC5m6n24Innd253U5s9KqZPpX9aYm0M6kaVFLWP5DzP1UVZsXJBu6Eb2aba6HjS30JKlGkyyPLlH2bZAAZeum8ZX4hiWj78fr7mWWG2auV2aRHnsrTqOTp5YnQpXzoRpZrCNjBuud0Quy7kq7HlebqM0srnAU9nEIORlKBlpV1row6KEQx38CQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cross-linking of [125I]CCL16 to HUVECs. HUVECs (2 × 107) were cross-linked with 0.5 nM [125I]CCL16 in presence or absence of 100-fold unlabeled ligand as detailed in “Materials and methods.” At the end of incubation, cell lysate was immunoprecipitated by a polyclonal Ab anti-CCR1 and protein separated by SDS-PAGE (10%). The figure is representative of 3 experiments performed.
