Cytosolic Ca2+ flux in triggered by CCL16 and modulation by BX471 compound. Cells were loaded with Fura2 and changes in fluorescence was monitored by a Perkin-Elmer LS-50B spectrofluorimeter. Panels A and B show Ca2+ transient stimulated by CCL16-Cos (Vec-Cos was negative; not shown) (A) or CCL11 (B) in HUVECs alone or preincubated with BX471 (15 minutes at 37°C). Panel C shows the effect of CCL16-Cos on KS cells (Vec-Cos was negative; not shown). Panel D shows the desensitization of HUVECs to a second stimulation with CCL16-Cos (50 ng/mL; black line) and the lack of desensitizing effect of CCL2 (50 ng/mL) on CCL16-Cos (50 ng/mL; orange line). Green lines indicate CCL16 + BX471 × 107 M; purple lines, CCL16 + BX471 × 108 M; and red lines, CCL16 + BX471 × 109 M. The line is interrupted exclusively to reduce the figure space. This is a representative experiment of 3 performed.
