Analysis of CCR1, CCR2, and CCR8 mRNA expression in HUVECs. RT-PCR was performed on RNA of HUVECs grown in complete medium by using specific primers and conditions indicated in “Materials and methods.” In the control sample, the reverse transcription reaction was not performed and RNA was used instead of cDNA. The size of amplification products of CCR1, CCR2, and CCR8 were respectively 360, 583, and 540 bp. The figure is representative of 3 experiments performed.