Figure 3.
Hepatic ECs motility triggered by CCL16. (A) Chemotaxis of liver ECs (2 × 105/50 μL) was evaluated as described in Figure 1. In some experiments cells were preincubated for 15 minutes with BX417. Mean ± SD of 3 experiments. Data were analyzed by one-way analysis of variance (F = 35.61) and Student-Newman-Keuls test. * indicates P < .05 versus Vec-Cos; §, P < .05 versus CCL16-Cos (50 ng). (B) CCR1 expression on the cell surface of liver ECs. Cells were incubated with either IgG control (black line) or with CCR1 mAb (gray line) and analyzed by flow cytometry. A representative experiment of 2 is shown.
