Amplification of HOX11L2 and HOX11 transcripts by real-time RT-PCR. Logarithmic representation of amplification plots obtained for patient samples are shown for HOX11L2 (A) and HOX11 (B). The normalized fluorescence signal (ΔRn) is plotted on the y-axis and the cycle number on the x-axis. Real-time RT-PCR permits to clearly distinguish patients with high HOX11L2 or HOX11 expression (low Ct), from those with low expression (high Ct) which were grouped with those negative for the statistical analysis.