![Figure 1. Activation of JNK and ERK in control and FA-A lymphoblasts. (A) Lymphoblast cell lines were exposed to UV radiation, cultured for 30 minutes, and subjected to immunoprecipitation with anti-JNK. The immunoprecipitates were then incubated with GST-ATF2 and [γ-32P]ATP. GST-ATF2 phosphorylation was assessed by SDS-PAGE and autoradiography (top panel). The lysates were analyzed by Western blot with anti-JNK (bottom panel). (B) Control and FA-A lymphoblasts were incubated in the presence of MMC for the indicated times and analyzed for the activity of JNK as assessed by phosphorylation of the GST-ATF2 substrate (top panel). The lysates were analyzed by Western blot with anti-JNK (bottom panel). (C) Control and FA-A cell lines were treated with PMA for 1 hour. Anti-ERK immunoprecipitates were subjected to immune complex kinase assays with MBP as substrate (top panel). The lysates were also analyzed by Western blot with anti-ERK (bottom panel).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/1/10.1182_blood-2003-06-2091/6/h80145411001.jpeg?Expires=1722767891&Signature=4-HusS~1BZ7bciN~HJstTBxwEqkdtyeUQBHhU-XuL0eA7PJmbQYW21jPPyHu6OM9IDT8lOu8Fk-9lFq2tGLLdAg5WzwTUbCsQu8qyz3Niv1GYUAtaAO3jYxl-cOHszCHquhORREa5rZMTnjIAQoCofFq21KkQuSfIGn7~NLtCWgNmO~x5849xNR8Xw5bA86nud8PrNTW7bKMYWwmu8xbwGx0O-Zj06ZlhJwZ6PFiwk2UrnG6q3Yg1THFm3pyAbLxiYF~f17LGi6xHIp8SxEC4X2GPfHmvPY~ERpkhTNC4LmnNNL-dH0lUmOeVdtLlXSTPKhLRFNWZJRd~yyA~k9VSw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of JNK and ERK in control and FA-A lymphoblasts. (A) Lymphoblast cell lines were exposed to UV radiation, cultured for 30 minutes, and subjected to immunoprecipitation with anti-JNK. The immunoprecipitates were then incubated with GST-ATF2 and [γ-32P]ATP. GST-ATF2 phosphorylation was assessed by SDS-PAGE and autoradiography (top panel). The lysates were analyzed by Western blot with anti-JNK (bottom panel). (B) Control and FA-A lymphoblasts were incubated in the presence of MMC for the indicated times and analyzed for the activity of JNK as assessed by phosphorylation of the GST-ATF2 substrate (top panel). The lysates were analyzed by Western blot with anti-JNK (bottom panel). (C) Control and FA-A cell lines were treated with PMA for 1 hour. Anti-ERK immunoprecipitates were subjected to immune complex kinase assays with MBP as substrate (top panel). The lysates were also analyzed by Western blot with anti-ERK (bottom panel).
