FISH detection of t(5;14)(q35;q32). Sequential hybridization of the 885A6 YAC (A), then of wcp14 (B) on the same metaphase. (A) The normal chromosome 5⁵ and the derivative 5 (der 5) are identified by green signals (885A6 YAC) at 5q35. (B) The derivative 5 is characterized by a small red signal (wcp14) at 5q35, corresponding to the translocated fragment from chromosome 14. (A) The derivative 14 (der 14) is identified by a green signal at its telomeric end (14q32), corresponding to the translocated part of the YAC. (B) The derivative 14 and the normal chromosome 14 are identified by wcp14 (red signal). Original magnification, × 100.