Microarray analysis of IL-6–regulated genes in INA-6 cells. INA-6 cells were cultured either in the presence of 1 ng/mL IL-6 (perm.) or deprived of IL-6 for 12 hours before restimulation with IL-6 for 1 hour or 4 hours, and expression patterns were studied using the Affymetrix U95A array. Two independent series of the experiment were carried out. Microarrays representing cells withdrawn from IL-6 for 12 hours were taken as baseline. Expression changes are indicated by the colors (red for induced and green for reduced expression) as shown.