Expression of CCL18 in Gaucher spleen. (A) Detection of CCL18 mRNA in Gaucher spleen by Northern blot analysis. Control and Gaucher spleen total RNA was analyzed by Northern blotting as described in “Patients, materials, and methods.” Lane 1, control spleen; lane 2, Gaucher spleen. The probes used were full-length CCL18 cDNA and GAPDH as an RNA control. The 18S ribosomal band is indicated. (B) Detection of CCL18 protein by immunohistochemistry in Gaucher spleen. Clustered large swollen cells are Gaucher cells and label massively for CCL18 protein (arrows). Some surrounding spleen cells also show some labeling (arrowheads). Original magnification × 400.