MCP-1–directed human monocyte migration. (A) Effect of anti–annexin II IgG. Monocytes (5 × 10⁵) were added to porous (8-μm) polycarbonate tissue culture inserts coated with Matrigel (MTG), after being incubated (45 minutes, 4°C) in the presence or absence of anti–annexin II tail peptide IgG (IM; 40 μg/mL; ± SE, n = 4), preimmune rabbit IgG (PI; 40 μg/mL; ± SE, n = 4). After 18 hours, plasminogen (PLG)–dependent migration in response to MCP-1 was expressed as percent of cells migrated under control conditions per × 40 field (*P < .01). (B) Effect of protease inhibitors. Monocytes (1 × 10⁵) were added to Matrigel-coated porous inserts in the presence or absence of amiloride (10 μM; Amil; ± range, n = 2) or α₂-plasmin inhibitor (50 μg/mL; α₂-PI; ± range, n = 2). After 18 hours, cell migration in response to MCP-1 was expressed as percent of control.