Monocyte plasmin generation. (A) Comparison of t-PA–dependent plasmin generated in the presence of suspended human peripheral blood monocytes (mono) and polymorphonuclear neutrophils (poly; 1 × 10⁶ cells/sample). Data collected over 90 minutes are shown as mean relative fluorescence units per minute squared (RFU/min²) ± SE, n = 5, P < .003. (B) Effect of ϵ-aminocaproic (ϵACA) on t-PA–dependent plasmin generation in the presence of human monocytes (Monos). Monocytes (1 × 10⁶) were incubated with or without ϵ-ACA (10 mM) in the presence of plasminogen (PLG; 170 nM) and t-PA (TPA; 12 nM). Shown are mean values ± SE, n = 3; *P < .04. (C) Effect of anti–annexin II IgG (60 μg/mL) on the t-PA–dependent generation of plasmin by monocytes. Cells were incubated in the presence of anti–annexin II polyclonal IgG directed against the t-PA–binding site (Anti-AII) or preimmune rabbit IgG (PI), as described in “Materials and methods.” Shown are mean values ± SE, n = 3; *P < .003. (D) Effect of anti–annexin II IgG (Anti-AII) versus preimmune IgG (PI) on u-PA–dependent plasmin generation by human monocytes. Shown are mean RFU/min² values expressed as percent of control (± SE, n = 3).