Corroboration of differential mRNA expression of selected probes by multiprobe RNase protection analysis. The same total RNA pools used for the gene array studies were analyzed for mRNA expression by RNase protection arrays (A). Five micrograms of each pool of RNA was used per hybridization to hCK1, hCK2b, and hStress multiprobe templates. Exposure times varied among the gels depending upon the strength of each signal. L32 mRNA is included as a control for input RNA. Representative results from 2 to 3 repeat experiments are shown. (B) Microarray expression data from the HG-U133A&B array corresponding to the above genes were graphed individually, showing the raw signal. ▪ indicates UCB; and •, adult.