Influence of EphB4 on cardiac myocyte differentiation. (A) Morphology of EB after attaching to gelatin-coated culture plates. (B) RT-PCR analysis of cardiac muscle–specific gene, α-cardiac myosin heavy chain (α-cardiac MHC). (C) EBs with beating cardiomyocytes. Paired data are linked by a line from the same experiment (n = 9). The thick line indicates the mean of 9 experiments.