Analysis of the DNA content of donor-derived hematopoietic cells and ECs in recipients of 500 KSL cells. Top left panel shows GFP⁺ donor-derived cells in a recipient liver by flow cytometry. Top right panel indicates cell sorting gates for GFP⁺, CD31⁺, CD45^– ECs and GFP⁺, CD31⁺, CD45⁺ hematopoietic cells. Sorted cell populations were fixed with ethanol and analyzed for DNA content with propidium iodide. Lower panels show propidium iodide uptake by sorted GFP⁺, CD31⁺, CD45^– ECs, GFP⁺, CD31⁺, CD45⁺ hematopoietic cells, and control normal spleen cells. The percentage of cells in S/G2/M of the cell cycle is indicated.