Induction of OFA-iLRP-specific CTLs by using autologous DCs transfected with OFA-iLRP RNA. DCs from 2 healthy HLA-A2⁺ individuals were transfected with OFA-iLRP RNA and used to stimulate CTL responses. Cytotoxic reactivity was determined in a 4-hour ⁵¹Chromium release assay against HLA-A2⁺/OFA-iLRP⁺ hematologic tumor cell lines (A), HLA-A2^-/OFA-iLRP⁺ targets (B), OFA-iLRP-transfected DCs (C), HLA-A2⁺ CD34⁺ PBPCs, (D) bone marrow cells from healthy individuals, and (E) HLA-A2⁺ EBV-transformed lymphoblasts transfected with OFA-iLRP RNA. One of 2 experiments is shown.