![Figure 1. Schematic description and localization of LMP1-GFP and Ub-LMP1-GFP expression constructs. (A) Four plasmids expressing LMP1 or LMP1 as a covalent fusion with the ubiquitin gene were generated in either pcDNA3.1 (LMP1; ubiquitin-LMP1 [Ub-LMP1]), or plasmid-enhanced green fluorescent protein-N1 (pEGFP-N1) (LMP1-GFP; Ub-LMP1-GFP) as described in “Materials and methods.” The LMP1/pcDNA3.1 constructs include insertion of an H-2Kd-restricted CTL epitope (VYGGSKTSL) at the LMP1 carboxy-terminal, which allows analysis of endogenous processing of LMP1. (B) GFP fluorescence of LMP1-GFP or Ub-LMP1-GFP-expressing Hela cells. Hela cells transiently transfected with pEGFP-N1, LMP1-GFP, or Ub-LMP1-GFP were examined by means of a laser-scanning Bio-Rad (Hercules, CA) MRC600 confocal microscope. Original magnification × 63.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/13/10.1182_blood-2003-03-0870/6/h82435405001.jpeg?Expires=1724234858&Signature=Gz4WveFATP2PAt77QcYkT8600osgBKmSMpM~aYA4BSsNVzOvricIXt2wmitQAH8LKVFpGKFLI0vQ2LAuXxOVy7CaYp7gMEA0~Es-M6Fi1cCfTcucWSvxBsTjzVVMM3czoYz02Oh3E3TnEiOl720822CW-0E7CHYXY5mNZx5ePd~oQ4Wv2KajPENb5VYUwdqF8qqrStuHfnttBVan8LGlwBW9YcriAaC93MKAxxO25d5wC9~NXeBYiqGa18dKrVec01sdaGC3BN3zqnC5ZfUxF9OFE22mHP0e7gHWYyGXvH4NYAXI5BYhe8slR-kdrCM4~p6fVBUp1ODPEta3j-Ipzg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Schematic description and localization of LMP1-GFP and Ub-LMP1-GFP expression constructs. (A) Four plasmids expressing LMP1 or LMP1 as a covalent fusion with the ubiquitin gene were generated in either pcDNA3.1 (LMP1; ubiquitin-LMP1 [Ub-LMP1]), or plasmid-enhanced green fluorescent protein-N1 (pEGFP-N1) (LMP1-GFP; Ub-LMP1-GFP) as described in “Materials and methods.” The LMP1/pcDNA3.1 constructs include insertion of an H-2Kd-restricted CTL epitope (VYGGSKTSL) at the LMP1 carboxy-terminal, which allows analysis of endogenous processing of LMP1. (B) GFP fluorescence of LMP1-GFP or Ub-LMP1-GFP-expressing Hela cells. Hela cells transiently transfected with pEGFP-N1, LMP1-GFP, or Ub-LMP1-GFP were examined by means of a laser-scanning Bio-Rad (Hercules, CA) MRC600 confocal microscope. Original magnification × 63.
