AML1-ETO-transduced CD34+ cells resemble an in vitro myelopoiesis culture. (A) Approximately 1 × 105 cells from a week-15 culture were stained with the indicated phycoerythrin-conjugated antibodies, and fluorescence was analyzed by flow cytometry. (B) Cytospins were performed using 8 × 104 cells, and slides were Wright-Giemsa-stained using standard protocols. Original magnification, ×600. (C-D) Long-term cultures of MIGR1-AE-transduced cells were stained for CD34 and sorted into CD34- and CD34+ cell fractions by fluorescent-activated cell sorter (FACS). The cells were then placed into liquid culture at 1 × 105 cells/mL. Cell surface expression of CD34 was determined by flow cytometry and cell expansion by trypan blue dye exclusion; both were performed weekly. Panel C is representative of 2 independent experiments, whereas panel D shows the average of 3 separate experiments.
