Figure 1.
AML1-ETO promotes the long-term in vitro expansion of human CD34+ cells. Human CD34+ cells were transduced with the MIGR1 or the MIGR1-AE retrovirus and expanded in culture using a mixture of human cytokines and 20% FBS or a serum substitute. Cells were counted by trypan blue dye exclusion weekly and replated at 4 × 105 cells/mL. Dagger indicates death of culture.

AML1-ETO promotes the long-term in vitro expansion of human CD34+ cells. Human CD34+ cells were transduced with the MIGR1 or the MIGR1-AE retrovirus and expanded in culture using a mixture of human cytokines and 20% FBS or a serum substitute. Cells were counted by trypan blue dye exclusion weekly and replated at 4 × 105 cells/mL. Dagger indicates death of culture.

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