EDN induces the activation of p44/42 MAPK in Mo-iDCs. Mo-iDCs untreated or treated with fMLP (10^-7 M) or EDN (1 μg/mL) for a period of time as specified were lysed, separated on a 4% to 12% gradient SDS–polyacrylamide gel electrophoresis (SDS-PAGE) gel, transferred to PVDF membrane, and Western blotted with anti–phospho-p44/42 (top). p, phosphorylated. The same membrane was stripped and blotted with anti-p44/42 (bottom). The exposure time for the top or bottom autoradiograph was 5 seconds. One experiment representative of 3 is shown.