Sensitivity of 2AA patient serum reactivity against GST-kinectin fusion protein. Equal amounts of bacterial lysates containing GST-full-length kinectin fusion proteins were separated by 7.5% SDS-PAGE, transferred to PVDF filters, and probed with patient 2AA sera (diluted at 1:250 to 1:5000) or normal serum diluted at 1:250. Immunoreactive proteins were detected with horseradish peroxidase-conjugated goat antihuman secondary antibody.