Figure 3.
Figure 3. Proliferative response of Kb-CD28-ζ and Kb-CD28[L→G]-ζ RMTCs. GFP-sorted CD8+ RMTCs were stimulated 7 days after transduction with irradiated splenocyte feeders on plates coated with AF6-88.5 anti-H-2Kb or in the presence of the nonspecific mitogen conA. After 2 days the cultures were pulsed with 3H-thymidine and harvested 16 hours later. Data points are means of triplicate samples. Error bars show ± 1 SD. One of 3 essentially identical experiments is shown.

Proliferative response of Kb-CD28-ζ and Kb-CD28[LG]-ζ RMTCs. GFP-sorted CD8+ RMTCs were stimulated 7 days after transduction with irradiated splenocyte feeders on plates coated with AF6-88.5 anti-H-2Kb or in the presence of the nonspecific mitogen conA. After 2 days the cultures were pulsed with 3H-thymidine and harvested 16 hours later. Data points are means of triplicate samples. Error bars show ± 1 SD. One of 3 essentially identical experiments is shown.

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