Chimeric receptor structure and sequence of the dileucine motif. Chimeric constructs were created by linking components in a cassette fashion. Extracellular and transmembrane domains are derived from the MHC class I H-2K^b molecule. The murine CD28 and TCR-ζ cytoplasmic tails were attached as described.¹⁰  PCR mutagenesis was used to introduce the leucine to glycine change in the CD28 tail. This corresponds to an L184G and L185G conversion in the CD28 sequence (GenBank accession NP_031668).