A phagocytophilum binds better to human PSGL-1 than to murine PSGL-1. (A) Flow cytometric analysis of binding of anti-human PSGL-1 mAb PL-1 or isotype-matched control mAb to transfected CHO cells expressing human PSGL-1. Bound mAb was detected with FITC-labeled goat antimouse IgG. (B) Flow cytometric analysis of binding of anti-murine PSGL-1 mAb 4RA10 or isotype-matched controls to transfected CHO cells expressing murine PSGL-1. Bound mAb was detected with FITC-labeled goat anti-rat IgG. (C) Flow cytometric analysis of binding of anti-sLex mAb HECA-452 or matched isotype control mAb to transfected CHO cells expressing either human or murine PSGL-1. Bound mAb was detected with FITC-labeled goat antirat IgM. (D) Flow cytometric analysis of CellTracker Green-labeled, PFA-fixed A phagocytophilum binding to nontransfected CHO cells, to murine PSGL-1-expressing CHO cells, to murine PSGL-1-expressing CHO cells pretreated with 4RA10, or to human PSGL-1-expressing CHO cells. Assays were performed in triplicate. Data are presented as the mean percentage (± SD) of cells bound by antibody or bacteria. Representative data from at least 2 studies are presented.
