Binding of A phagocytophilum to Fuc-TIV^-/-/Fuc-TVII^-/- murine neutrophils is severely reduced. CellTracker Green-labeled, PFA-fixed A phagocytophilum were incubated with leukocytes from (A) wild-type and (B) Fuc-TIV^-/-/Fuc-TVII^-/- mice. Bacterial binding was directly correlated to CellTracker Green fluorescence using flow cytometry. In each panel, a thick line indicates neutrophils; and a thin line, lymphocytes. Assays were performed in triplicate. Data are presented as the mean percentage (± SD) of leukocytes bound by bacteria. Representative data from at least 2 studies are shown.