Effect of R115777 on the induction of apoptosis in 3 myeloma cell lines. Cells were cultured with R115777 at a concentration of 1.0 × 10^-8 M. After incubation for 0, 12, 24, 48, and 72 hours, cells in 500 μL cultured suspension were washed and stained with fluorescein isothiocyanate (FITC)-labeled annexin V (AN) and 7-amino-actinomycin D (7AAD) at each time point and analyzed with a FACS Calibur flow cytometer. R115777 at 1.0 × 10^-8 M induced apoptosis in the U266 (A) and NCI-H929 (B) cell lines time dependently but did not induce apoptosis in the RPMI 8226 cell line (C). ▦ indicates viable; ▪, early apoptosis; and □, late apoptosis and necrosis.