American Society of Hematology

Figure 5.

$Figure 5. Apoptosis in mutant stem cells. BM cells were harvested 4 weeks after induction, stained, and analyzed by FACS. (A) Lineage marker-positive cells and dead cells (7AADhi) were excluded (R2), and Sca1/c-kit–positive cells were selected (R3). (B) The gated population was subsequently analyzed for Annexin V staining. In this example, negative control cells without Annexin V are shown by the black histogram, and cells stained with Annexin V are shown in gray. (C) The fraction of apoptotic cells (Annexin Vhi, M1 in Figure 5B) from TβRI null and control mice are shown (n = 7 per genotype). There was no significant difference between TβRI null and control (P = .5). Data represent means ± SDs.$

Apoptosis in mutant stem cells. BM cells were harvested 4 weeks after induction, stained, and analyzed by FACS. (A) Lineage marker-positive cells and dead cells (7AAD^hi) were excluded (R2), and Sca1/c-kit–positive cells were selected (R3). (B) The gated population was subsequently analyzed for Annexin V staining. In this example, negative control cells without Annexin V are shown by the black histogram, and cells stained with Annexin V are shown in gray. (C) The fraction of apoptotic cells (Annexin V^hi, M1 in Figure 5B) from TβRI null and control mice are shown (n = 7 per genotype). There was no significant difference between TβRI null and control (P = .5). Data represent means ± SDs.

This Feature Is Available To Subscribers Only