Amelioration of spleen, liver, and kidney pathology in lenti/β^AS3mice.(A) Spleen, liver, and kidney sections were analyzed at low (original magnifications are × 10 for spleen and kidney and × 40 for liver; top 3 rows and bottom row) and high magnification (original magnification, × 100; bottom rows for spleen and liver and the middle row for kidney). In lenti/β^AS3-transduced mice, normal splenic red and white pulp is observed and virtually no pools of sickle erythrocytes or infarcts are evident. In livers of lenti/β^AS3 animals, focal areas of necrosis and aggregation of sickled erythrocytes are not observed; also, extramedullary hematopoiesis and hemosiderin deposition are absent. Kidneys of lenti/β^AS3 mice appear normal and free of the disruptive vascular RBC pooling and hemosiderin deposits observed in mock-treated animals. (B) Correction of splenomegaly in lenti/β^AS3-transduced mice. All sections except the bottom panel were stained with hematoxylin-eosin; the bottom panel was stained with Gomori iron.