Isolation of highly purified Sca-1⁺c-Kit+Lin- HSCs from sickle mice.WBM was sorted on a MACS column to obtain Sca-1-enriched cells. Three-color preparative FACS was then performed to isolate Sca-1⁺, c-Kit+, Lin-/low HSCs. Purified KLS cells satisfy gating requirements for R1 (A), R2 (D), and R3 (C). R4 (B) indicates the c-Kit⁺ and Lin-/low cell population.