AML-1/ETO represses CD11a integrin promoter activity: dependency on the AML-110 element. (A) K562 cells were transfected with 1 μg of the indicated reporter plasmids and in the absence or in the presence of a CMV–AML-1/ETO expression plasmid. In all cases, total DNA was kept constant by the addition of empty CMV-0 plasmid DNA. For each individual reporter construct, “fold induction” represents the luciferase activity produced by each individual expression plasmid relative to the activity in the presence of empty CMV-0 plasmid. The experiment was performed 4 times using distinct DNA preparations. Data represent mean ± SD of the 4 experiments (*P = .004 and P = .0001 when comparing the activity of pCD11A170-Luc and pCD11A170(–100mut)-Luc in the presence or absence of the CMV–AML-1/ETO expression plasmid). (B) HeLa cells were transfected with 1 μg of pCD11A170Luc reporter plasmid and in the absence or in the presence of either CMV–AML-1 (RUNX1) and/or CMV–AML-1/ETO expression plasmids. In all cases, total DNA was kept constant by the addition of empty CMV-0 plasmid DNA. For each individual reporter construct, “fold induction” represents the luciferase activity produced by each individual expression plasmid combination relative to the activity produced by a similar amount of empty CMV-0 plasmid. Data represent mean ± SD of 3 experiments using distinct DNA preparations (*P = .03 compared with the activity of the wild-type pCD11A170-Luc construct in the presence of RUNX1).