![Transplantation of BM cells in OSMR+/+ mice. OSMR+/+ or OSMR–/– GFP-positive BM cells were transplanted into busulfan-treated neonatal OSMR+/+ mice. +/+ (+/+) indicates OSMR+/+ mice reconstituted with BM cells of GFP-transgenic OSMR+/+ mouse; +/+ (–/–), OSMR+/+ mice reconstituted with BM cells of GFP-transgenic OSMR–/– mouse; and –/–, GFP-transgenic OSMR–/– mice. Recipient mice were killed 4 months after transplantation and the levels of donor contribution in peripheral blood were determined by FACS. Four +/+ (+/+) mice exhibiting relatively high proportion of donor-type and 2 +/+ (–/–) mice (nos. 1 and 2) exhibiting high chimerism were used for the following hematologic analyses. Orbital plexus blood was collected from anesthetized mice. Peripheral RBCs (A) and platelets (B) were analyzed by using automated counter Sysmex K-4500. The horizontal bar represents the mean. (C-D) The hematopoietic progenitor content (GM-CFCs, left panel; and CFU-GEMMs/CFU-MEs/BFU-Es, right panel) of the BM (C) and spleen (D) cells were assessed. The figure represents the mean ± SD [n = 4 for +/+ (+/+) and –/–] and each value for +/+ (–/–).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/9/10.1182_blood-2003-02-0367/6/h82135157007.jpeg?Expires=1720293595&Signature=q8Rv9bg5SWlEFRpU0IGf8PoJTdNJB24b3BeISL3fbhcKlcjB44lXnnmxN3F7zDxn~-sj8lp8zQI4UWsvY9issVS~UjQClm3WRGKndQkXp5yggy07JV-Puq62QBlijJTEU-mO3hGWsrP662mRUVA~0YlwR6fuOjrzwhNBOa~nTOjA3PrO5mVL9HK98QgOUuHv-6fSHRa005uVZi3MqmRX3ghvdCWlJNZWZ9ZEzdRB22oxI2jZ2c3cqfrkGVjowpZu6d7ECyJbWU-h~WwjpirMg2JciWeJI-faHNiN6722CBiW~VW8vqegGVpy7zn~U0oJyD5~Y4a9~9rPGDrRWDVZWw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transplantation of BM cells in OSMR+/+ mice. OSMR+/+ or OSMR–/– GFP-positive BM cells were transplanted into busulfan-treated neonatal OSMR+/+ mice. +/+ (+/+) indicates OSMR+/+ mice reconstituted with BM cells of GFP-transgenic OSMR+/+ mouse; +/+ (–/–), OSMR+/+ mice reconstituted with BM cells of GFP-transgenic OSMR–/– mouse; and –/–, GFP-transgenic OSMR–/– mice. Recipient mice were killed 4 months after transplantation and the levels of donor contribution in peripheral blood were determined by FACS. Four +/+ (+/+) mice exhibiting relatively high proportion of donor-type and 2 +/+ (–/–) mice (nos. 1 and 2) exhibiting high chimerism were used for the following hematologic analyses. Orbital plexus blood was collected from anesthetized mice. Peripheral RBCs (A) and platelets (B) were analyzed by using automated counter Sysmex K-4500. The horizontal bar represents the mean. (C-D) The hematopoietic progenitor content (GM-CFCs, left panel; and CFU-GEMMs/CFU-MEs/BFU-Es, right panel) of the BM (C) and spleen (D) cells were assessed. The figure represents the mean ± SD [n = 4 for +/+ (+/+) and –/–] and each value for +/+ (–/–).
