Figure 1.
Targeting disruption of the mOSMR gene. (Panel A) Targeting strategy of the mOSMR gene. (i) Schematic diagram of the wild-type allele. The start codon is indicated by ATG. The open box represents the location of the probe used to detect the restriction fragment of genomic DNA by Southern blot analysis. Restriction enzyme sites are indicated as ApaI (A), EcoRI (E), EcoRV (V), HindIII (H), BglII (B), and SacII (S). (ii) Gene targeting vector. The vector contains the 5′ and 3′ regions of homology and the cDNAs encoding neomycin transferase (Neo), LacZ, and diphtheria toxin (DT-A). (iii) Diagram of the targeted mutant OSMR allele. (Panel B) Southern blot analysis of the EcoRV-digested genomic DNA extracted from the OSMR+/+, OSMR+/–, and OSMR–/– mice. (Panel C) Northern blot analysis of neonatal lung mRNA (+/+, +/–, and –/–) with mOSMR probe.

Targeting disruption of the mOSMR gene. (Panel A) Targeting strategy of the mOSMR gene. (i) Schematic diagram of the wild-type allele. The start codon is indicated by ATG. The open box represents the location of the probe used to detect the restriction fragment of genomic DNA by Southern blot analysis. Restriction enzyme sites are indicated as ApaI (A), EcoRI (E), EcoRV (V), HindIII (H), BglII (B), and SacII (S). (ii) Gene targeting vector. The vector contains the 5′ and 3′ regions of homology and the cDNAs encoding neomycin transferase (Neo), LacZ, and diphtheria toxin (DT-A). (iii) Diagram of the targeted mutant OSMR allele. (Panel B) Southern blot analysis of the EcoRV-digested genomic DNA extracted from the OSMR+/+, OSMR+/–, and OSMR–/– mice. (Panel C) Northern blot analysis of neonatal lung mRNA (+/+, +/–, and –/–) with mOSMR probe.

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