CTL recognizing the CYP190 or CYP239 peptide can be generated from cancer patients and healthy donors. (A) After 4 ex vivo antigenic stimulations, CTLs raised from healthy HLA-A*0201 donors against CYP190 or CYP239 peptide specifically lysed T2 cells pulsed with 20 μg/mL cognate peptide (▪) but not unpulsed T2 cells (□) or T2 cells pulsed with an irrelevant peptide (○, F271 from MAGE-3). (B) Cytotoxicity of CYP190-specific (•) and CYP239-specific (♦) CTLs against T2 cells pulsed with increasing concentrations of cognate peptide (effector-target ratio, 10:1). Dashed lines reflect the peptide concentration at which half-maximal lysis was achieved. Results are representative of 2 independent experiments.